This study investigates biogas production from co-digestion of cornstalk with goat dung and its statistical analysis. Physicochemical analysis of substrate and digestate were also carried out as well as microbial count load analysis. Result shows highest experimental biogas yield of 4.7 × 10-2 m3, temperature of 38oC on 29th day. The predicted biogas yield by the model was 4.44 x 10-2 m3, temperature of 37.7 °C on 29.0895th day. Using the optimum condition of 29.0895th day to load two digesters, an average biogas yield of 4.216 x 10-2 m3 and mesophilic temperature of 37 oC was attained, which was well within the range predicted by the model. Analysis of variance (ANOVA) of regression equation shows that the coefficient of determination R2 of 73.34% and 55.60% were obtained for biogas and temperature during the analysis. The temperature of the slurry was found to be within the mesophilic temperature ranges (30-40 oC). The pH of the slurry decreased from 7.95 to 7.55 in the space of 4 weeks. Physicochemical analysis of substrate and digestate varies. Hence, it can be concluded that alkali treated dried cornstalk (ATDCS) with goat dung is a good waste material for biogas production and the by-products or spent slurry of this process could also be used as fertilizer or improved organic manure for agricultural production.