Abstract Serum malondealdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and histological analysis of lungs were carried out in twenty rats exposed to smoke from transfluthrin impregnated insecticide paper. The rats were divided into five groups (I, II, III, IV and V) of four each. Group one served as control while Group II, III, IV and V were exposed to smoke from transfluthrin impregnated paper Insecticide for 15, 30, 60, 120 minutes daily for four weeks respectively. On the 29th day all the rats in the five groups were euthanized and blood sample was collected and centrifuged for analysis of biochemical parameters (MDA, AST, ALT and ALP), the animals were dissected and tissue from the lungs were collected for histological studies. Significant increase (p<0.05) in all the parameters were observed in time dependent manner when compared with control. However, histopathological analysis of the lung tissue shows no pathological changes. Thus, the chemical in the transfluthrin impregnated insecticide paper can modify biochemical parameters but within the context of duration of this research, no significant impact in lung tissue was observed. However, transfluthrin impregnated paper insecticide should be used with caution even at lower doses.