Abstract Three simple, accurate, sensitive, selective and cost effective spectrophotometric methods were developed and validated for quantitative determination of miconazole nitrate (MIC) and hydrocortisone (HDC) in pure form, laboratory prepared mixtures and topical pharmaceutical preparation without any preliminary separation step. HDC was determined directly at λmax 242.6 nm in the presence of MIC, while MIC was determined by three spectrophotometric methods namely; isoabsorptive point, ratio subtraction and ratio difference. In isoabsorptive point spectrophotometric method, the isoabsorptive point (Aiso) at 231 nm was chosen for determination of MIC. The second method was ratio subtraction at which MIC was determined at its λmax220 nm after subtraction of interference exerted by HDC using 12 μg mL-1 of HDC as a divisor. While in ratio difference method, MIC was determined by dividing the recorded spectra by a 12 µgmL-1of HDC as a divisor then measuring the difference in peak amplitude values between 211 and 230 nm for determination of MIC. The developed methods were found to be linear in the range of (2 – 24 μg mL-1) with correlation coefficient 0.9999, and found to be linear in the range of (4 – 24 μg mL-1) with correlation coefficient 0.9999 for MIC and HDC respectively. The developed methods were validated according to ICH guidelines. The results of the three methods were statistically compared to those obtained by the official method for determination of the cited drugs and showed that the proposed methods were accurate, reliable and precise as the reported one.
Chemisty Research Journal
A Peer Review International Journal
Chemisty Research Journal © 2016
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