Abstract This paper study Comparative analysis of Moringa oleifera seed oil using alternative solvents (Petroleum ether and Acetone). The influence of different conditions and physicochemical parameters using petroleum ether and acetone were analysed statistically. The results of the physicochemical properties for petroleum ether and acetone are as follows; Peroxide value: (1.16 mEq/kg and 0.84 mEq/kg), free fatty Acid value: (1.14 mgKOH/g and 1.08 mgKOH/g), Saponification value: (200.67 mgKOH/g oil and 253.32 mgKOH/g oil), Iodine value: (86.03 mgiodine/100g and 85.35 mgiodine/100g), Specific gravityL 0.91 and 0.90) respectively. The coefficient of determination (R2) value obtained from ANOVA showed that petroleum ether is a good alternative solvent to acetone.
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