Preliminary Pharmaceutical Constituents of Crude Solvent Extracts of Flower and Stalk of Male Carica papaya

Abstract Qualitative and quantitative phytochemical screening of flower and stalk of male Carica papaya indicated the presence of these phytocompounds: alkaloids 0.53±0.01%flavonoids 0.86±0.02%, saponins 0.37±0.02%, tannins 2.06±0.01%, terpenoid 0.21±0.01%, steroid 0.08±0.01% and cardiac glycoside 1.87±0.02%. The ten fungi investigated in this research are: Aspergillus niger, Microsporum gypseum, Candida albican, Aspergillus flavus, Fusarium verticilloids, Aspergillus parasiticus, Fusarium oxysporum, Candida glabrata, Candida parasilosis, Candida tropicalis. Three solvents were used in the extraction of active ingredients in flower and stalk of make Carica papaya and they are: distil water, ethanolic and N-hexane. Aqueous extract of flower and stalk of male Carica papaya cannot inhibit two out of ten test fungi. These are: Fusarium verticilloides and Fusarium oxysporum. Ethanolic extract cannot inhibit three out of ten test fungi. These are: Fusarium verticilloides, Aspergillus parasiticus and Fusarium oxysporum. N-hexane extract cannot inhibit four out of ten test fungi. These are: Microsporum gypseum, Aspergillus flavus, Fusarium verticilloides and Fusarium oxysporum. The minimum inhibitory concentrations (MIC) of ten fungi investigated as mentioned above using three solvents are: aqueous extract, 0.05±0.01mg/ml, 9.35±0.02mg/ml, 0.05±0.02mg/ml, 0.05±0.02mg/ml, no inhibition, 0.05±0.01mg/ml no inhibition, and 0.05±0.01mg/ml, 0.05±0.01mg/ml and 0.05±0.01mg/ml respectively. The MIC for ethanolic extract are: 0.25±0.01mg/ml, 0.25±0.03mg/ml, 0.05±0.01mg/ml, 0.05±0.01mg/ml, no inhibition, no inhibition, no inhibition, 0.05±0.01mg/ml, 0.05±0.01mg/ml and 0.25±0.03mg/ml respectively. The MIC for n-hexane extract are: 0.45±0.03mg/ml, no inhibition, 0.05±0.01mg/ml, no inhibition, no inhibition, 0.05±0.01mg/ml, no inhibition 0.05±0.01mg/ml, 0.05±0.01mg/ml and 0.05±0.01mg/ml respectively. The minimum fungicidal concentration (MFC) for ten test fungi as mentioned above are: for aqueous extract 0.04±0.01mg/ml, 0.30±0.01mg/ml, 0.02±0.01mg/ml, 0.05±0.01mg/ml, no inhibition, 0.05±0.01mg/ml, no inhibition, 0.05±0.03mg/ml 0.5±0.01mg/ml and 0.03±0.01mg/ml respectively. The MFC for ethanolic extract are: 0.15±0.03mg/ml, 0.25±0.01mg/ml, 0.02±0.01mg/ml, 0.05±0.02mg/ml, no inhibition, no inhibition, no inhibition, 0.03±0.01mg/ml, 0.03±0.01mg/ml and 0.05±0.01mg/ml. The MFC for n-hexane extract are: 0.23±0.02mg/ml, no inhibition, 0.02±0.02mg/ml, no inhibition, no inhibition, 0.03±0.01mg/ml, no inhibition, 0.02±0.01, 0.05±0.02mg/ml and 0.05±0.01mg/ml respectively. Hence, crude solvent extracts of flower and stalk of male Carica papaya are antibiotic in nature.

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