Abstract Four selected Nigerian plants namely Azadirachta indica (neem), Garcinia kola (bitter cola), Gongronema latifolium (heckel) and Vernonia amygdalina (bitter leaf) as potential substitutes for hops in beer brewing were evaluated in terms of methanolic extracts. Phytochemical screening of the extracts was carried out using standard methods. The iso-alpha acid and essential oil contents of the extracts were investigated using UV-visible spectrophotometer. Phytochemical results showed that alkaloid content in all the extracts ranged between 3.2-4.8%; tannin ranged from 2.0-4.8%; saponin ranged from 0.80-5.20%; haemagglutini ranged from 3.879-7.240%. The concentration of iso-alpha acid ranged from 7.95-12.53ppm and essential oil content ranged from 0.544g/100g to 1.012g/1`00g. Consequently, the extracts from tested Nigerian plants could be used as suitable substitutes for hops in beer brewing.