Abstract Fruits are the main fruit trees grown throughout the world and are well–appreciated for their refreshing juice and health benefits. This study of Citrus paradisi (peels) of hexane, acetone and methanol extracts was carried out to show phytochemical screening and antibacterial activity. Plants and plant products are continuously being explored in medicine against the increasing number of antibiotic resistant organisms. The antibacterial activity of plant under study has been done by suing agar well diffusion method against bacteria. Phytochemical analysis showed the presence of terpenoids , alkaloids , flavoniods , tannins , reducing sugars and glycosides. Citrus paradisi extracts at a concentration of 100 mg/ml was determined by agar well diffusion method against pathogenic microorganisms; (Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, and Staphylococus aureus). The crude extract of Citrus paradisi was very active against Bacillus subtilis strains (IZ = 25 mm zone of inhibition). This study has shown that grape peels contain some antibiotic principles which may be explored for use in the treatment of certain diseases.