Metabolites Composition of Garcinia kola Extract as Potential Substitute for Isomerized Hop Extract in Beer Brewing

Abstract The potential of methanoilc extract of Garcinia kola (bitter cola) as substitute for isomerized hop extract in beer brewing was evaluated. The number of metabolites in each extract was investigated using Thin Layer Chromatography (TLC) technique. The relative proportion of each metabolite in the two extracts was investigated using Gas Chromatography – Mass Spectrometer (GC-MS). The TLC results revealed that the extract of G. kola contains 12 metabolites while isomerized hop extract contains 14 metabolites. The GCMS results were in agreement with the results of the TLC with respect to the number of metabolites in each extract. The results of the GCMS also showed that Garcinia kola extract contains some metabolites comparable to those of isomerized hop extract, although some metabolites [dehydro-cohumulunic acid; 4,4-dimethyl-2-buten-4-olide; 1,2-dimethyl-cyclopropane carboxylic acid; lupulone; 2,5-dimethyl-2-hexanol; 4,4,5,5-tetramethyl-bicyclo hexyl-6-ene-2,3-dione; octadecanoic acid, oxiranyl methyl ester and 1,2-benzenedicarboxylic, bis(-2-ethyl hexyl) ester] present in isomerized hop extract were absent in Garcinia kola extract. Consequently, academic activity in the area of mixtures/blends of extract of plant species which mimic isomerized hop extract is strongly recommended.

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Updated: June 25, 2023 — 7:47 am