Abstract Diabetes is a leading cause of morbidity and mortality in the world. There is currently an active search for antidiabetic drugs with greater effectiveness with fewer and less adverse side effects. Therefore, in present study the hypoglycemic potential of polyherbal formulation was investigated in alloxan induced diabetic rats. A list of medicinal plants with proven antidiabetic and related beneficial effects of herbal drugs used in treatment of diabetes is compiled. These include Gymnema sylvestre, Syzygium cumini, Trigonella foenum- graecum, Azadirachta indica, Momordica charantia, Curcuma Longa, Piper Nigrum, Terminalia Bellirica, Swerita Chirayita and Paneer Pera. The formulated polyherbal preparation was further examined for its glucose tolerance and antidiabetic activity in rats. Antidiabetic effect was analyzed in alloxan- induced diabetic rats of the polyherbal formulation. Glibenclamide was used as the standard drug. The percentage yield, phytochemical parameter of the polyherbal formulation was assessed. Polyherbal formulations in Alloxan induced diabetic rats showed significant decrease in blood glucose.